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KMID : 0603820100160020127
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Journal of Experimental & Biomedical Science
2010 Volume.16 No. 2 p.127 ~ p.131
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Detection of Mycobacterium leprae by Real-time PCR Targeting Mycobacterium leprae-Specific Repetitive Element Sequence
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Jin Hyun-Woo
Wang Hye-Young
Kim Jong-Pill
Cho Sang-Nae
Lee Hye-Young
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Abstract
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Mycobacterium leprae detection is difficult even with molecular biological techniques due to the low sensitivity of current methodologies. In this report, real-time PCR targeting the M. leprae-specific repetitive element (RLEP) sequence was developed as a new diagnostic tool and evaluated using clinical specimens. For this, M. leprae DNAs were extracted from skin biopsy specimens from 80 patients and analyzed by real-time PCR using TaqMan probe. Then, the detection efficiency of the real-time PCR was compared with that of standard PCR. In brief, the rate of positive detection by the standard PCR and real-time PCR was 32.50% and 66.25%, respectively. The results seemed to clearly show that the TaqMan real-time PCR developed in this study may be a useful tool for sensitive detection of M. leprae from clinical specimens.
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KEYWORD
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Mycobacterium leprae, Real-time PCR, Repetitive element sequence (RLEP)
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